Description：
Aneuvin BTXA 50iu is botulinum toxin type A, the highly purified protein from bacteria(Clostridium botulinum) in GMP (Good Manufacturing Practice) pharmaceutical factory with the strictest quality control. Injection of slight doses of botox into specific facialmuscles can significantly remove facial wrinkles.
Base information：
WHAT IS BOTULINUM TOXIN A ?
Botulinum toxin type A(BTXA) is the highly purified protein from bacteria(Clostridium botulinum) pharmaceutical factory with the strictest quality control. Injection of slight doses of this toxin into specific facialmuscles can significantly remove facial wrinkles.
Specifications: 50iu/vial
Shelf Life: 3 years in freeze-drying conditions
Storage: Store at temperature of -5 to -20°C ，Maximum solution temperature 36°C.
Please be aware that there are duplicated goods on the market
Each vial contains:
Botulinum toxin typa A -------50iu
Albumin-----------0.5mg
Dextran------------30mg
Mannitol------------30mg
NaCl-----------------0.9mg
Application：
Botulinum Toxin A is used for the removing facial lines, including:
（1）glabellar (frown) lines frontal lines smile lines,
（2）blepharospasm, hemifacial spasm
（3）crow's feet etc.
（4）some types of strabismus

Storage and Transportation：
（1）Freeze-dried products in -20 ℃ can be kept for 3 years , in 2-8 ℃ ,then 2 years, in 25 ℃ ,7-10 days .
（2）Therefore, If the BTXA is packed with dry ice or Jelly ice during transport, BTXA will not lose the effect for 7-10days.

Dilution method:
1 remove drugs from the refrigerator when you decide the treatment..
2 After taking it out , put at room temperature for a certain time, usually 10-15min, in order to be similar as the room temperature,then easy to dilute.
3. Generally diluted 50iu.with 2-4ML normal saline .
4. when dilution, you should stick the bottle wall safety with the syringe needle then push the Normal saline into bottle slowly to avoid air bubbles, make all the powder drugs dissolved, Don’t use excessive force. Otherwise ,it will affect the drug action.
5. In the melting process, avoid repeated suction to reduce vibration and prevent bubble formation, bubble will make protein denaturation ,which can affect drug action.
6, configured solution should be used immediately or placed in refrigerator 2-8 ℃, 4h in good condition, no more than 6h, if keep too long ,will reduced efficacy. After 12h it will be 44% inactivation ,1-2week, 70% inactivation .
7 BTXA injection should be sufficient quantity one time , injection intervals must be more than 3 months. BTXA is protein, if low-dose, short interval will be easy to form antibody which make a small effect, even invalid.

Injection Tips:
In order to reduce the complication of blepharoptosis:
(1)Avoid injection near the levator ，palpebrae superioris, particularly in patients with larger brow-depressor complexes
(2)Medial corrugators injections should be placed at least 1 cm above the bony supraorbital ridge
(3) usage dose ：It is about 25 iu best for Cosmetic injection ,not more than 100iu.
(4) It is the best 0.1ML (2.5-5 iu ) for each injected point.

Detail Information：
How is the Product Made？
BTXA Injectable (Botulinum Toxin Type A for therapy) is a sterile, lyophilized form of purified . botulinum toxin type A, was produced from the crude toxin of the culture of the Hall strain of Clostridium botulinum grown in a medium containing trypticase and yeast extract.
A series of purifying procedures are taken to form a crystalline complex consisting of the active high molecular weight toxin protein and an associated hemagglutinin. After re-dissolving and dialyzing the crystalline toxin, an accurate amount of the sterile filtered (0.2 microns) ，toxin is added to a solution containing gelatine-dextran-sucrose, then lyophilized.

Product Contents and Consistency
Generally，Each vial of BTXA contains 100 units (U) of botulinum toxin , 5mg of gelatine, 25mg of dextran and 25mg of sucrose. Dilute with sterile normal saline according to the different needs before using. The white loose production turns to be a colourless or yellowish transparent solution after the reconstitution. One unit (U) of BTXA corresponds to 1 LD50 of Botulinum Toxin Type A being intraperitoneally injected into a laboratory mouse. BTXA could block neuromuscular conduction by inhibiting the release of acetylcholine and therefore cause local muscle flaccid paralysis.

Clinical studies
Cosmetic and dermatological application of in China
Clinic Application of Botulinum Toxin A in Removing Wrinkles for 20 patients
Botulinum toxin used in children strabismus
Treatment of focal dystonia and muscle spasm
Contraindications:
（1）BTXA is contraindicated in individuals with anaphylactic tendency and hypersensitivity to this preparation.
（2）The injection should be postponed in patients who have fever, acute infectious diseases and administered more carefully to patients with blood, heart, liver, lung diseases, active tuberculosis, pregnant women and never to children below 12 years of age.
（3）BTXA must not to be given to those who suffers from eyebrow hyperplasia and myasthenia gravis, it is ineffective in patients with serious muscle fibre contracture.
Adverse reactions ：
Included pain on the face, erythema at the injection site, and muscle weakness will be infrequence ，less than 3%. They can be avoided by appropriate injection techniques and mostly occur within the first week.
Botulinum Toxin A is minimally invasive and no recover time is required. Rightly after treatment the patients can go back to work. So there is no downtime, and it will not disturb your normal life.

Side effects ：
There is no noticeable side effects in all injected patients even long term repeatedly receiver. Some local adverse reactions might occur in small amount of patients. Like Allergan ，Botulinum toxin type A, The most common side effects are headache, respiratory infection, flu syndrome, temporary eyelid droop, and nausea. However, side effect is mild, temporary and will disappear naturally within 2-8 weeks.

Question：
1.How does BTXA work?
Normally, your brain sends electrochemical messages to your muscles to make them contract and move. These messages are transmitted from a nerve to the muscle by a substance called acetylcholine. When too much acetylcholine is released, muscles become overly active and spasm or tense up.
BOTULINUM TOXIN TYPE A blocks the nerve from releasing acetylcholine. As a result, the muscle spasms stop or are greatly reduced, providing relief from symptoms. Your health care provider will know how much BOTULINUM TOXIN TYPE A is needed to treat you effectively.
It's important to remember that botulinum toxin treatment is not a cure. For many people, however, its effects have been dramatic. With BOTULINUM TOXIN TYPE A， the nerve will take about 3 months to recover and begin to release acetylcholine, and the muscles may become overactive again. At that point, another injection will be needed to provide relief, as long as no allergic reactions or other significant side effects occurred and clinical response was obtained.
2.How Long can I be treated with BTXA ?
（Each treatment typically lasts up to 3 months and can be repeated as long as your condition responds to BOTULINUM TOXIN TYPE A and you do not have any serious allergic reactions or other significant side effects. BOTULINUM TOXIN TYPE A has been used for more than 11 years to treat more than 1 million patients worldwide, and although formal, long-term clinical evaluations have not been conducted, its safety in long-term use has been well established.
Although most people continue to respond to BOTULINUM TOXIN TYPE A injections, some people have experienced a diminished response over time. There may be several explanations for this:
Changes in your condition - If the pattern of your muscle activity changes, your health care provider may need to inject new muscles and/or change your dose. Identifying and injecting the affected muscle can be difficult, complicated by the changing pattern of muscle involvement and progression of the disorder.
Setting appropriate expectations - You may believe your first BOTULINUM TOXIN TYPE A injection was more helpful than subsequent injections. That's because your condition was perhaps quite severe when you had your first injection. Subsequent injections are usually given before your condition becomes that severe again. Therefore, the relief you experienced with subsequent injections may not have been as dramatic as the first time.
Antibody formation - When foreign proteins, like botulinum toxins, enter your body, antibodies may form. If antibodies to botulinum toxin develop, you may no longer respond to treatment.
Because botulinum toxins are usually used to treat chronic conditions, it's important to preserve responsiveness to therapy.
3.How can I help maintain my response to BTXA ?
While the critical factors for neutralizing antibody formation have not been well characterized, you may be able to help maintain your response to BOTULINUM TOXIN TYPE A by minimizing your total exposure. The potential for antibody formation may be minimized by injecting with the lowest effective dose given at the longest feasible intervals between injections

4.How is BTXA given?
BOTULINUM TOXIN TYPE A is injected into the affected muscle(s). Your doctor will determine which muscles need to be treated.
5.Does the injection hurt?
Some people report minor, temporary discomfort from the injection. BOTULINUM TOXIN TYPE A is reconstituted with sterile, preservative-free normal saline for injection. The neutral pH of the injected solution, in combination with the fine-gauge needle your doctor will use, can help to minimize any injection-related pain.
6.When will BTXA start to work?
If you're receiving BOTULINUM TOXIN TYPE A for cervical dystonia, you'll usually see the effects within 2 weeks of the injection. If you're receiving BOTULINUM TOXIN TYPE A for blepharospasm, you'll usually see effects within 3 days.
Initial effect is different in different injection location on different body. Normally it is around 1 week.

7.How Long will the effect last?
BOTULINUM TOXIN TYPE A offers sustained relief, dose after dose. The relief you'll feel from one treatment of BOTULINUM TOXIN TYPE A will normally last for up to 6-12 months. Treatments can be continued as long as your condition responds to BOTULINUM TOXIN TYPE A, and you do not have any serious allergic reactions or other significant side effects. When the relief begins to fade, you'll return to your doctor for your next treatment.
Usually, BOTULINUM TOXIN TYPE A treatment is required approximately four times per year. Because symptoms can change over time, the amount and duration of relief you'll experience can vary. Consult your doctor, who can determine how to achieve the best possible results with BOTULINUM TOXIN TYPE A
8.Is BTXA right for me?
Your health care provider can help you decide if BOTULINUM TOXIN TYPE A is right for you. In order to make the right treatment decision, you should discuss the following with your health care provider before choosing treatment:
Clinical experience with the drug
Effectiveness and side effects
Make sure your health care provider knows if you are pregnant, nursing, or taking any medications before receiving BOTULINUM TOXIN TYPE A injections. Additionally, you should not receive BOTULINUM TOXIN TYPE A if you have an infection at the injection site.
BOTULINUM TOXIN TYPE A should be used with caution if you have other neurological diseases or disorders, or if you are taking amino glycoside antibiotics or other drugs that interfere with neuromuscular transmission. Be sure to tell your health care provider about any prescription or over-the-counter medications you are taking before receiving BOTULINUM TOXIN TYPE A.

Before & After Treatment Pictures